Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β-lactamases

CTX-M Extended-spectrum β-Lactamases, Washington State

Our study of risk factors for T. gondii infection was a case-control design to evaluate recent infection, not a cross-sectional study of T. gondii infection prevalence in a population. In our study, case-patients with recent infection were similar in age to T. gondii–negative control-patients, although among women the mean age of case-patients (33 years) differed slightly from that of control-p...

Detection of CTX-M-β Lactamases form Isolated Klebsiella pneumoniae

  Background and Objective: Organisms producing CTX-M β-lactamases are emerging as a source of resistance to oxyiminocefalosporins such as ceftriaxone and ceftazidime. However, the laboratory detection of these strains is not well defined. In this study, phenotypic assay for screening of extended-spectrum β-lactamases producing strains and molecular assay for the identification of CTX-M β-lact...

Direct RNA-Based Detection and Differentiation of CTX-M-Type Extended-Spectrum β-Lactamases (ESBL)

The current global spread of multi-resistant Gram-negatives, particularly extended spectrum β-lactamases expressing bacteria, increases the likelihood of inappropriate empiric treatment of critically ill patients with subsequently increased mortality. From a clinical perspective, fast detection of resistant pathogens would allow a pre-emptive correction of an initially inappropriate treatment. ...

Molecular Basis for the Catalytic Specificity of the CTX-M Extended-Spectrum β-Lactamases

Extended-spectrum β-lactamases (ESBLs) pose a threat to public health because of their ability to confer resistance to extended-spectrum cephalosporins such as cefotaxime. The CTX-M β-lactamases are the most widespread ESBL enzymes among antibiotic resistant bacteria. Many of the active site residues are conserved between the CTX-M family and non-ESBL β-lactamases such as TEM-1, but the residue...

Growing Group of Extended-Spectrum -Lactamases: the CTX-M Enzymes